"Introduction to Capillary Electrophoresis"
Robert Weinberger, Ph.D.
Chappaqua, NY USA
This lecture will provide for an introduction to capillary electrophoresis from a phenomenological viewpoint. We will examine just how ions move about in fluid solution based on charge and the impact of the surrounding environment. You will gain an appreciation for the basis of electrophoresis and understand the impact of experimental variables on the e-gram. Some of the various modes of CE including zone electrophoresis, isoelectric focusing, size separations and electrokinetic chromatography will be described along with their underlying mechanisms. Sample preparation, injection and detection is covered highlighting differences between CE and liquid chromatography. Slides will be provided via flash drive.
New Advances in CE-MS for Metabolomics and Biomarker Discovery: Expanding Throughput and Metabolome Coverage with Quality Control
Dept. Chemistry and Chemical Biology, McMaster University
This short course is aimed at presenting the major technical hurdles associated with biomarker discovery in metabolomics research when using capillary electrophoresis-mass spectrometry (CE-MS). The history of metabolomics and the use of biomarkers in clinical medicine or epidemiological research will first be described, including criteria needed to be satisfied for their qualification. Recent advances in metabolomics technology based on CE-MS will next be presented for the discovery of unknown biomarkers of clinical significance, including robust study designs, data workflows and statistical approaches to reduce false discoveries and bias. The unique advantages of validated CE-MS protocols in metabolomics research will also be presented, including recent breakthroughs to enhance sample throughput, expand metabolome coverage and identify unknown compounds with stringent quality control. Case studies will be discussed as related to non-targeted drug screening of clinically depressed patients, the impact of strenuous exercise and physical inactivity on metabolic health, as well as the validation of biomarkers reflecting habitual dietary patterns.
Microchip-electrophoresis coupled to nanomaterial-based electrochemical detection in food analysis
Flavio Della Pelle
Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo
64100, Teramo, Italy
Nowadays, microfluidic technology applied to food analysis has become a valuable tool able to solve food safety and quality issues. This meeting between food analysis and microfluidic, result more and more promising because can allow obtaining a fast quali-quantitative analysis in a chip. In a parallel track, over the last twenty years, nanomaterials (NMs) have made their entry in the analytical chemistry domain; NMs have, in fact, opened new paths for the development of analytical methods with the common aim to improve analytical performance thanks to their modulable features, becoming elective tools able to allow the realization of unique devices. Thus, this communication aims to overview the fascinating coupling between the microchip-electrophoresis ant the electrochemical detection based on nanomaterials, paying particular attention to the applications in food. The ultimate goal of this communication is to demonstrate how the 'NM-based microchip-electrophoresis' approaches represent a valid and unique technology able to allow novel and unexpected achievements, and it is mature to be integrated for the rapid food quality and safety assessment in-lab or directly in 'field/factories'.